Mrs. Bolton was the teacher of Deadpool and Bullseye when they sat in the same class together. However, both kids were revolting and kept insisting on wearing masks. When Bolton's punishments weren't enough for the boys to change their minds, Bolton threatened to call their dads. Bullseye got so scared about this and killed the teacher, and he and Deadpool later burned down their school together, deciding to become evil that day.
Mrs. Bolton appears in the following comics:
Deadpool (volume 3) #12